Most of poly aromatic hydrocarbons are stable in marine environment and don’t degrade easily. If they accede to the aquatic animals bodies, they will accumulate in their fat tissues. The toxic effects of these compounds are consisting of carcinogenic, mutagenic and germinal tribulation. Hence present study was done with purpose of isolation and identification of naphthalene degrading bacteria and comparison of their ability to eliminate this substance. Therefore naphthalene resistant bacteria were isolated and then purified after sampling of oil polluted sediments, implementation of enrichment process and culturing on MSM medium. Among 8 obtained bacterial species 2 of them were selected for further studies as the most capable naphthalene degrading species because of their higher optical density and lower medium pH after 3 days of inoculation. It was characterized that these species were P. aeroginosa and P.putida with perusal morphological trait and accomplishment of some biochemical test. Substrate depletion by these species was measured by HPLC. Results showed degradation measure were 96.14±0.662% and 91.48 ±1.1501% by P. aeroginosa and P. putida respectively after 120 hours of incubation. These values are considerable in comparison with 14.598±3.735% degradation in the same condition but with no bacterial inoculation. Overall results showed isolated bacteria are able to eliminate this pollutant from medium and they presumably have similar demeanour in natural environment so they can be suggested for outside experiments and used in high impurity condition and inoculated in polluted site in higher quantity in exigency.